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KMID : 0363320140350020175
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Journal of Korean Oriental Internal Medicine
2014 Volume.35 No. 2 p.175 ~ p.183
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Research on Anti-lipogenic Effect and Underlying Mechanism of Laminaria japonica on Experimental Cellular Model of Non-alcoholic Fatty Liver Disease
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Kim So-Yeon
Kwon Jung-Nam
Lee In
Hong Jin-Woo
Choi Joon-Yong
Park Seong-Ha
Kwun Min-Jung
Joo Myung-Soo
Han Chang-Woo
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Abstract
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Objectives : We tried to uncover the anti-lipogenic effect and underlying mechanism of Laminaria japonica on an
experimental cellular model of non-alcoholic fatty liver disease.
Methods : Ethanol extract of Laminaria japonica (LJ) was prepared. Intracellular lipid content of palmitate-treated HepG2 cells was evaluated with or without LJ treatment. We measured the effects of LJ on liver X receptor α (LXRα) and sterol regulatory element-binding transcription factor-1c (SREBP-1c) expression, transcription level of lipogenic genes, including
acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and nuclear factor erythroid 2-related factor 2 (Nrf2) activation in HepG2 cells.
Results : LJ markedly attenuated palmitate-induced intracellular lipid accumulation in HepG2 cells. LJ suppressed LXRα
-dependent SREBP-1c activation, and SREBP-1c mediated induction of ACC, FAS, and SCD-1. Furthermore, LJ activated
Nrf2, which plays an important cytoprotective role in non-alcoholic fatty liver disease.
Conclusions : Our study suggests that LJ has the potential to alleviate hepatic lipid accumulation, and this effect was
mediated by inhibiting the LXRα-SREBP-1c pathway that leads to hepatic steatosis. In addition, the anti-lipogenic potential
may, at least in part, be associated with activation of Nrf2.
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KEYWORD
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Laminaria japonica, non-alcoholic fatty liver disease, LXRα, SREBP-1c, Nrf2
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